Graticules, Stage Micrometers, and Calibration Standards Eyepiece Graticules (continued)


G. Stereology


In this simplest form, sterology is the science where information about a three dimensional object is obtained from only a two-dimensional section of that structure.


Measurements are usually made with these graticules in the following manner: 1. An adequare representation of sections of a specimen is obtained. 2. The graticule is superimposed upon the specimen (or micrograph/projected image of the section). 3. Finally, the interaction between the superimposed graticule and the test sections are recorded


An overall introduction is given by: L.B. Brianrty. “Stereology: Methods for Quantitative Light and Electron Microscopy.” The Mertz Graticule (36 Points) — NGM1


Used to estimate the three-dimensional surface areas or the surface density of a component in a given volume, when the component does not have a random orientation. It comprises a test system with parallel curved lines used for measuring the intersection of points.


Reference: W.A. Mertz. "Mikroskopic" Vol. 22 1967 pp 132-142. Pattern


Description NGM1


Mertz for Stereology. Surface chrome image.


Weibel 1 – NGW1


Consists of 15 lines of equal length connecting the verticals of a regular hexagonal point network.


Reference: E.R.Weibel Lab. Invest. Vol. 22 pp 131-152. "Principal and Methods for the Morphometric Study of the Lung and other Organs."


Pattern NGW1


Description


Weibel Type 1 for Stereology. Surface chrome image.


Weibel 2 – NGW2


Used when making a surface to volume ratio of a structure per mass unit. This graticule consists of a number of short lines with interruptions as long as the lines. Basically, the number of intersections falling over the short lines is counted and the number of endpoints falling on the end of the structure is determined.


Reference: E.R. Weibel, Journal of Microscopy Vol. 95. pp 373-378. Current Capabilities and Limitations of Available Sterrological Technique, point counting method.


Pattern NGW2


Description


Weibel Type 2 for Stereology Surface chrome image.


Dia.


16mm 19mm 21mm


Cat.#


68025-16 68025-19 68025-21


Dia.


16mm 19mm 21mm


Cat.#


68022-16 68022-19 68022-21


Dia.


16mm 19mm 21mm


Cat.#


68035-16 68035-19 68035-21


30


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