EMS-002


Rapid Immersion Freezer Overview


Ultra-rapid freezing, when used in conjunction with appropriate accessory processing methods has long been recognized to provide reservation of ultrastructure, localization of soluble inorganic and organic species, and preservation of antigenicity that is superior to that obtainable through conventional chemical fixation techniques and the use of cryoprotectants. Furthermore, it is the only procedure currently available for capturing events that occur on timescales less than a few seconds. Successful ultrarapid freezing requires that heat be withdrawn so rapidly from an unfixed un-cryoprotected sample that no ice crystal damage is visible at the electron microscopic level of analysis. This is not a trivial undertaking, and until recently, the improvements gained through ultrarapid freezing were often paid for in terms of decreasing productivity. However, over the last several years, improvements in methodology and the introduction of refined equipment for the ultrarapid freezing of biological samples has significantly improved both the ease of application of technique and the yield of well preserved samples. The EMS-002 Rapid Immersion Freezer allows for a reliable virtually routine freezing of biological samples.


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1. Removable Drop Anvil Assembly 2. Specimen Holder 3. Stainless Steel Liquid Nitrogen Dewar 4. Cryogenic Temperature Controller 5. Drop Release 6. Removable Cryogen Holder for safe disposal of cryogen


7. Frozen Specimen Workstation Applications


z Cryo-fixation of virus partials z Cryo-fixation of isolated or assembled macromolecules z Cryo-fixation of emulsions paints and polymers


z Cryo-fixation of suspension and tissues for Cryo-techniques such as freeze fracture/etch, freeze drying, freeze substitution.


Advantages z Economical, universal application rapid freezing unit. z Plunge freezing with temperature controlled cryogen. z Frozen samples handled under cold dry nitrogen atmosphere. z Rapid set up and easy use. z Reproducible results. z High and low set points for cryogen control.


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