NEWS
Introducing new cryo-EM workflow solutions
Linkam, a market leader in temperature and environmental- controlled microscopy, presented its range of cryo workflow solutions and sample characterisation tools at Microscopy and Microanalysis (M&M) 2022, where it previewed its new cryo plunger – CryoGenium – an exciting development in electron microscopy (EM) grid vitrification.
Imaging of biological samples embedded in vitrified ice has become of great interest in recent years as it provides several advantages: i) the biological sample is in a fully hydrated state with superior preservation down to ultrastructural level; ii) a vitrified sample is naturally compatible with the vacuum required for EM/single particle (SPT)/ correlative light and electron microscopy (CLEM); and iii) cryo-fluorescence provides very low photo-bleaching and high signal- to-noise imaging. Preparation and handling of vitrified samples normally requires special skills and techniques. The novel design of the Linkam CryoGenium makes this a simple and reproducible process. CryoGenium addresses several common issues that are found in a conventional plunge-freezing set-up. Typically, the sample is prepared on an EM grid and excess liquid is removed inside a humidity chamber using blotting paper to control the ice thickness of the frozen sample. The design replaces the common blotting mechanism to improve process stability and repeatability. The system introduces real-time optical observation of the sample with control of the liquid film thickness by adjusting the speed at which the sample is drawn from the fluid as well as thinning of the film thickness by controlled
Anti-human IgE antibodies for serology and diagnostics
suction. The correct conditions for plunging can be confirmed by the real-time optical observation, and plunging is followed by automated loading into either a special cryo-holder or a cryo-transfer container. CryoGenium provides a platform that
increases the success rate and quality of plunged grids, enabling improved efficiency and less time wasted at transmission EM (TEM), resulting in cost savings. The Linkam CMS196V3 Cryo-Correlative
Stage provides another cryo correlative tool enabling the full workflow of CLEM to maximise efficiencies in the laboratory. Cryo-CLEM is a rapidly emerging technique that combines the advantages of fluorescence light microscopy (FLM) with three dimensional (3D) cryo-electron tomography (cryo-ET) to reveal the ultrastructure of target molecules with specific cellular functions. As well as maintaining the integrity of the sample vitrified by liquid nitrogen cooling, the Linkam CMS196V3 stage enables contamination-free sample handling and transfer for optical microscopy imaging.
www.linkam.co.uk
Jackson ImmunoResearch has announced a new addition to its range of products for scientists developing immunoassays for the serology and diagnostics markets. Jackson ImmunoResearch (JIR) mouse monoclonal anti-human IgE antibodies are the latest addition to the company’s suite of products suitable for diagnostics research and development. Specific for human IgE antibodies, they complement existing anti-human IgG, IgM, and IgA antibodies. Anti-human IgE is available conjugated to a range of reporter molecules, including alkaline phosphatase and biotin, enabling excellent sensitivity for immunoassay applications.
Jackson ImmunoResearch anti-human IgE is a mouse-derived monoclonal antibody with reactivity to human E class immunoglobulins and is epsilon (ε) chain-specific and has been tested by enzyme-linked immunosorbent assay (ELISA) and shows minimal cross- reactivity to other classes of human immunoglobulin (IgG, IgM or IgA). Anti-human IgE is available conjugated to a range of reporter molecules and is suitable to detect human IgE by various techniques, including ELISA, lateral- flow immunoassay, flow cytometry, Western blot, and chemiluminescence immunoassay. More information on Jackson ImmunoResearch mouse monoclonal anti-human IgE antibodies is available online.
www.jacksonimmuno.com/ige
New Blackpool histopathology laboratory officially opened
Blackpool Teaching Hospitals received a VIP guest of honour early in August, when Dr Michael Osborn, president of The Royal College of Pathologists, officially opened the trust’s new histopathology laboratory. With dedicated sections for a range
of different laboratory activities, the new facility boasts bespoke specialist equipment, more adequate space for the department’s workload and overall is a vast
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improvement in working environment for the trust’s dedicated histopathology team. The new laboratory will provide better facilities for staff, but, more importantly, increase the capacity to diagnose diseases and conditions, including cancers. For a virtual tour of the new laboratory, visit the link below.
https://my.matterport.com/ show/?m=yct5x9Yq46R
SEPTEMBER 2022
WWW.PATHOLOGYINPRACTICE.COM
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