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Gilts are more sensitive to Beauvericin than sows


BY DR REGIANE SANTOS, MYCOTOXINS RESEARCHER AT SCHOTHORST FEED RESEARCH B


eauvericin (BEA) is a Fusarium mycotoxin that is known for its antiviral, antibacterial, anti-inflammatory, and anticancer properties, but it also causes oxidative stress and cell death. Although these biological activities are mainly interesting


for drug development, chronic animal exposure to such a chemical via feed will certainly influence performance and health status. Al- though this mycotoxin is still ‘emerging’, its presence in feed materials has been known for many years.


Beauvericin is commonly found as a co-contaminant in grains where other Fusarium mycotoxins such as Deoxynivalenol (DON) and Zear- alenone (ZEA) are present. From the feedstuffs we evaluate at SFR, BEA is often detected in corn and soy hulls at levels varying from 10 to 500 µg/kg. However, much higher contamination levels have been reported by others. As was published by All About Feed in 2010, a study performed in South Korea showed that 27% of feed ingredients were then contaminated with BEA at levels up to 1.8 mg/kg (almost four times higher than our findings). These levels can be extremely high, reaching circa 500 mg/kg in corn as was reported by Logrieco and others in the 1990s. Although these are extreme levels and they only occur occasionally, the constant presence of this mycotoxin is feedstuffs should be of concern to nutritionists and veterinarians.


In collaboration with Utrecht University, the Netherlands, we have been performing in vitro trials to evaluate the effect of several myco- toxins on the fertility of gilts and sows. For this, we apply a validated in vitro culture method of gametes that can be further in vitro


Figure 1 - Percentages of viable produced embryos from in vitro matured oocytes after 


% Produced embryos from oocytes 100 75 50 b 25 c 0 0   Beauvericin (μg/mL)  a a


fertilised for embryo production. In the first trials, we studied the ef- fects of BEA on gametes harvested from sows and it was clear that sows can cope with the oxidative stress caused by exposure to BEA at levels up to 4 µg/mL. However, when performing another study with gametes collected from gilts, exposure to five times lower concentra- tions of BEA (0.8 µg/mL) resulted in impaired embryo development, i.e. 50% fewer embryos produced in vitro (Figure 1).


To understand the effects observed in these two studies, we simulta- neously exposed gametes from gilts and sows to BEA, and the results confirmed our concerns. Gilt gametes were not able to mature, while 66% of the gametes from sows matured in vitro when they were ex- posed to 4 µg/mL BEA. It is known that the developmental potential of sow oocytes is higher than that of gilt oocytes because the antioxi- dant capacity of sow gametes is higher than in gilts. This difference in oxidative stress resistance may play a role in sensitivity to BEA.


Due to the small number of studies on the exposure of farm animals to this mycotoxin it is difficult to compare plasma levels with the levels tested in our culture system. However, these levels were similar to those previously detected in the plasma from rats after oral exposure via feed. Most importantly, in practice gilts will be subject to chronic exposure via feed and BEA can accu- mulate in fat tissues. There is cur- rently no data on the adverse ef- fects of BEA in pig farms and such analysis is difficult because die- tary exposure consists of a mixture of various Fusari- um mycotoxins including DON, ZEA, and Enniatins, among others. This is why our studies with cells draw attention to the risks of ne- glected mycotox- ins such as BEA, in- dicating that special attention should be paid to diets offered to gilts that are contaminated with BEA.


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