in-cos Cat 30/3/09 17:58 Page 60
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The warm rays of sunlight improve the circulation of
the blood in our skin and organs, and stimulate the
formation of Vitamin D, making us feel better, more
attractive and have more energy
Protection against damage
However, sun can also be dangerous. Sun rays are composed of visible Methods and Results
light, infra-red and ultra-violet (UVA and UVB) rays. Whereas UVA can Detection of DNA damage in 3D epidermal model
cause indirect DNA damage through the creation of reactive oxygen species by CELLnTEC
(ROS), UVB directly damages DNA e.g. through the formation of thymine Human Epidermal Keratinocyte Progenitors (HPEK) from pooled donors
dimers (TT-dimers). The cell must then be repaired in order to survive. were used to establish 3-D models. Models were grown for 16 days before
Severely damaged cells can no longer be repaired as the energy needed for treatment for 30 minutes with either CELLIGENT
®
, Placebo or left untreated.
regeneration cannot be made available in a short period of time. Cells The 3D cultures were then irradiated with a controlled UV dose of 1500
which cannot regenerate vital cell functions die off. mJ/cm2 (UVA&UVB: MEGA-RAY SB100W) for 15 minutes. Models were
Photoageing of the skin is the main consequence of UVA radiation. UVB then sampled at 1h, 4h and 24h post irradiation, and analysed for the
radiation often triggers an acute skin inflammation known as sunburn. The presence of thymine dimers using immunohistochemistry and a TT-dimer
consequence of DNA damage caused by UVB is the synthesis of different specific antibody (Figure 1).
proteins and enzymes. The effects of these proteins, notably prostaglandins
and cytokines, lead to dilatation of cutaneous blood vessels and recruitment
of inflammatory cells. This, in turn, produces sunburn’s characteristic
redness, swelling and pain. When the first signs of sunburn appear, the dose
of UV radiation which can be accommodated has already been exceeded
many times over, and the skin’s repair mechanisms are overloaded.
CELLIGENT
®
protects the DNA and supports the repair of UV-induced
cell damage. Three active principles are interlocked: a) neutralisation of
dangerous oxygen radicals using natural antioxidants, b) reduction of the
dose of UV radiation using ingredients with UV-absorbing properties, c)
support of DNA repair using a biochemical pre-stage of nucleotides
required for this process. Figure 1: 3D model at 1, 4 and 24 hours post UV exposure, stained for
thymine dimers (dark brown). Prior to exposure, models were
treated with either CELLIGENT
®
, Placebo or left untreated. No
staining was observed in the CELLIGENT
®
treatment
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