MICROPLATE READERS
and size (diameter). During a typical run, a droplet of sample on a carrier slide is illuminated from below and imaged while being moved vertically (Figure 1). Each analysis returns size
and contrast measurements for every particle, making it possible to determine the size-contrast distribution. This enables users to characterise multiple populations within a sample and assess sample purity and stability. Developed by Refeyn Ltd., macro
mass photometry is a rapid, reliable and convenient approach to inform process development and optimisation for vaccine and CGT development. Macro mass photometry is performed in an all-in-one benchtop instrument that delivers results in several minutes. In the analysis of vectors, the technology can be leveraged to
inform: ●
Relative population counts
What are viral vectors? Viral vectors are designed to deliver genetic material into cells and are one of
the most eff ective methods of gene therapy. Viruses have evolved to develop mechanisms that insert their genomes inside the cells they infect. Modifi ed viruses are used as viral vectors (or ‘carriers’) in gene therapy, protecting the new gene from degradation while delivering it to the ‘gene cassette’ in target cells.
(correlating with physical and infectious titer)
●
Particle morphology (size distribution)
● Sample purity ● Stability and degradation
DETERMINING ADV SAMPLE PURITY Adenovirus (AdV) characterisation can be challenging due to the coexistence of various particle types in the process. Before downstream
characterisation can be challenging due to the coexistence of various particle types in the process.
❝Adenovirus
Refeyn’s Two auto mass photometer enables rapid, autonomous, and label-free mass characterisation of biomolecules at a single molecule level.
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Figure 1: Macro mass photometry - how it works
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