PEER-REVIEW | FACIAL AESTHETICS |
PRP preparation Draw blood (4 ml for each tube), then
centrifuge tubes at 2000 rpm for 5 minutes. Take the syringe, insert the needle and withdraw 0.5 ml DNA Activator (10% calcium chloride). Withdraw platelets and mix with the DNA Activator. Multiple injections (0.05–0.1 ml for a single injection)
were applied to the intra/sub dermis using the Ômulti-prickingÕ or retrograde linear techniques
Omnilux 633 nm LED This was applied for 20 minutes per session (126 J/cm2
).
Phase 3 (Week 3) Low-level UltraPulse Lumenis ActiveFX with settings (energy) 100 mJ (rate) 14 w CPG 3/5/2.
Omnilux 633 nm LED This was applied for 20 minutes per session (126 J/cm2
).
Histology Skin biopsies were obtained from five of the patients intra-operatively, before Phase 2 of the treatment and at 3 months post-operatively, and were performed to determine the amount of epidermal damage, subsequent inflammation, and new collagen synthesis. The extent of neocollagenesis was compared with data on file for patients who had skin biopsies for laser resurfacing and neurotoxin alone in 2007. Each 1 cm by 1 cm piece of skin was fixed with 10% formalin solution, neutral buffered. After treatment with polyester wax, the skin samples were sliced into 6 m m thicknesses. The sliced sections were treated with haematoxylin and eosin statin
Figure 6 Cachexic patient with volumisation post PRP/ DUBLiN Lift
(H&E) and MassonÕs trichrome staining solutions. Through tissue evaluations, the thickness of the dermal layer and presence of collagen fibres were observed. The thickness of the dermal layer was calculated by measuring five different sites from each section, and the mean value of the thickness of the dermal layer for each group was used for the comparison.
Results Over 3 months, 29 subjects (Group 2) were selected to compare the effect of low energy fractional laser skin resurfacing with adjunctive treatments to conventional ablative laser resurfacing. These patients received a three-phase combination of established treatments with neurotoxin, microneedling, platelet growth hormones, near-red 633 nm light, and low-energy UltraPulse fractional CO2
laser skin tightening over a 3-week period. Phase 1
included the administration of Dysport– neurotoxin to the upper face. Phase 2 introduced fibroblast stimulation from microneedling and PRP growth factor induction with near- red phototherapy, and Phase 3 included low-level (CO2
) Over 3 months, 29 subjects
(Group 2) were selected to compare the effect of low energy fractional
laser skin resurfacing with adjunctive treatments to conventional ablative laser resurfacing.
UltraPulse laser with adjunct near-red 633 nm phototherapy. Results were compared to the remaining 15 patients (Group 1) who received fractional laser skin resurfacing (125 mJ; 19 w CPG 3/5/4), and whose data was already on file. Patients in both groups were administered Dysport– neurotoxin 1 week prior to treatment to complement and preserve the overall aesthetic effect. The study evaluated post-procedural aesthetic results at baseline, 6 weeks and 12 weeks by means of a scoring system based on DoverÕs photoageing scale, as well as using the WSRS and GAIS.
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January/February 2013 |
prime-journal.com
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