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Table of Contents


Chapter 6 Monolithic Cryogel Beds Prepared Under Varying Freezing Temperatures and Their


Application in the Isolation of Nucleotides 127 Kejian Yao, Junxian Yun and Shaochuan Shen 6.1 Introduction


6.2 Freezing Characteristics of a Monomer Solution


6.3 Preparation of a Cryogel Matrix by Varying the Freezing Temperature


6.6 Isolation of Nucleotides From Crude Fermentation Broths by Anion-exchange Chromatography Using Cryogels


6.7 Conclusions Acknowledgements References


Chapter 7 Organic Monoliths as Stationary Phases in Chromatography


Andreas Greiderer, S. Clark Ligon Jr., ChristianW. Huck and Gu¨ nther K. Bonn 7.1 Introduction


7.2 Organic Monolithic Stationary Phases 7.5 Characterisation of Organic Monoliths


127 129


6.4 Monolithic Cryogel BedsWith Embedded Nanoparticles 131 6.5 Ion-exchange Monolithic Cryogel Beds


130 133


134 136 137 137


139


139 141


7.3 Chromatographic Characteristics of Monolithic Polymers 147 7.4 Pore Formation and Porous Properties of Organic Monoliths


153 161


7.6 Chromatographic Applications of Organic Monoliths 164 7.7 Summary and Conclusions References


Part II Applications of Monolithic Columns


Chapter 8 Applications of Monolithic Chromatography in Pharmaceutical Analysis


Debby Mangelings and Yvan Vander Heyden 8.1 Introduction


8.2 Types of Monoliths and Their Preparation


8.3 Pharmaceutical Applications of High-performance Liquid Chromatography


8.4 Pharmaceutical Applications of Capillary Liquid Chromatography


177 177


179 182 200


170 171


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