Table of Contents
Chapter 12 Fast Separations of Phytochemicals by High- performance Liquid Chromatography Using Monolithic Columns
Mauricio A. Rostagno and Ana Villares 12.1 Introduction
12.4 Practical Aspects and Considerations for the Use of Monolithic Columns
12.5 Method Development Strategies Using Monolithic Columns
12.6 Future Potential of Monolithic Columns in Liquid Chromatography
References
Chapter 13 Monolithic Stationary Phases for Affinity-based Separations
Panagiotis Manesiotis and Georgios Theodoridis 13.1 Introduction
13.2 Monolithic Materials for Affinity Chromatography
ix
339 339
12.2 Chromatographic Characteristics of Monolithic Columns 341 12.3 Applications of Monolithic Columns for the Analysis of Phytochemicals
343 366 368 376
377
381 381
13.3 Applications of Monoliths in Affinity Chromatography 387 13.4 Molecularly Imprinted Polymers 13.5 Concluding Remarks References
Chapter 14 Immobilisation of Enzymes on Monolithic
Matrices: Applications in Drug Discovery 403 Vincenza Andrisano and Manuela Bartolini 14.1 Introduction
14.2 Enzyme Immobilisation on Monolithic Matrices
403 405
14.3 Determination of Enzyme Activity After Immobilisation 412 14.4 Kinetic Characteristics of the Immobilised Enzyme 14.5 Enzyme Inhibition 14.6 Conclusions References
415 417 423 423
383 392
399 400
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