38 BIOTECHNOLOGY
be automated for major gains in efficiency and time savings.
Te new flat LED powered Leica TL5000 Ergo defines new standards in reproducibility and automatic contrasting. Te homogenous field of view with its 65 mm diameter is optimised for single cells to whole animal examinations.
Diffuse light
Te base has an integrated zoom dependent automatic aperture, which exclude diffuse light in brightfield to increase contrast. By displacing the light source out of the beam paths, perfect oblique illumination in Rottermann contrast appears.
Furthermore, each contrast mode is coded and automatised, resulting in complete reproducibility and full controllability via the Leica LAS or LAS AF software.
Te transmitted-light bases truly play out their strengths when combined with their accessories. Te
Leica mechanical stage, for example, noticeably simplifies day-to-day laboratory work with its low profile and precise X and Y control.
Heating stages, adapters for micromanipulators and the entire Leica Live on Stage product line open a full range of applications that the company says were previously beyond the capabilities of stereomicroscopes.
Finally, the new Leica DFC365 FX digital microscope camera combines exceptional image quality with very high temporal resolution for rapid time-lapse recordings.
Te Leica DFC365 FX is setting new standards in its class enabling researchers to work efficiently, even with weakly fluorescing specimens.
Equipped with a highly sensitive CCD sensor (pixel size 6.45 µm) and active Peltier cooling, it is ideal for a wide range of applications – from basic fluorescence documentation to demanding experiments such as
total internal reflection fluorescence (TIRF), fluorescence resonance energy transfer (FRET) or structured illumination.
Te Leica DFC365 FX achieves acquisition rates of 21 fps at full resolution .
Image acquisition In ‘overlapping mode’, an image can be captured while the previous image is still being read out. Data are transferred rapidly to the PC via a FireWire-B interface. Besides high-speed image acquisition at 40 MHz, the pixel clocking rate of the sensor can also be set to 20 MHz or 1.6 MHz as required. According to the company this yields brilliant fluorescence images with a superb signal-to-noise ratio.
Te optional NIR (near infra-red) mode extends the operating range of the camera for fluorescence markers emitting in the wavelength range above 700nm, which are difficult to capture with conventional CCD technology.
“The new Leica DFC365 FX digital microscope camera combines exceptional image quality with very high temporal resolution for rapid time-lapse recordings. The Leica DFC365 FX is setting new standards in its class enabling researchers to work efficiently, even with weakly fluorescing specimens.”
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