CONTINUING EDUCATION TEST • JUNE 2023 Sponsored by
Positive biological indicators Circle the one correct answer:
1. Which of the below statements about biologi- cal indicators (BI) is/are true? A. A positive BI result should be investigated to determine the cause.
B. The purpose of a BI is to identify when microorganisms are not killed.
C. A BI contains a known number of microor- ganisms.
D. All of the above.
2. Which of the below measures do NOT contrib- ute to the performance characteristics of BIs? A. D-values B. Survival and kill values C. Cost D. Spore population
3. What does the acronym CFU stand for? A. Conditions for use B. Colony forming unit C. Critical failure underkill D. None of the above
4. What requirements need to be met for a BI to be an appropriate challenge for the steriliza- tion process? A. Contain microorganisms with a high resistance to the mode of sterilization being monitored
B. Contain at least 1x106 CFU for ethylene oxide sterilization processes
C. Contain at least 1x105 CFU for steam steril- ization processes
D. All of the above CONTINUING EDUCATION TEST SCORING
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5. A marginal sterilization cycle ___. A. successfully kills all microorganisms B. can yield both positive or negative BI results C. is never due to human error D. All of the above
6. Sterilization cycles are designed to kill all spores at which point in the cycle? A. 25% B. 50% C. 90% D. 100%
7. Which of the following factors could contrib- ute to a sterilization process failure? A. Sterilizer malfunction B. Inadequate steam quality C. Selection of the incorrect cycle for the load D. All of the above
8. Which of the following statements is/are NOT true about rapid readout BIs? A. Rapid readout BIs are less reliable than slow- read BIs.
B. Rapid readout BIs contain an enzyme substrate attached to a fl uorescent indicator dye.
C. Rapid readout BIs can detect biologically active spores without needing to wait for the spores to multiply.
D. All of the above
9. What is the AAMI ST79 and AORM recom- mended frequency for monitoring steam ster- ilization cycles with a BI in a process challenge device? A. At least weekly, preferably daily, plus im- plants
B. At least monthly, preferably weekly, plus implants
C. Only after a sterilization process failure D. None of the above
10. Which of the following statements are true about monitoring with BIs? A. BI testing does not need to be done in both gravity-displacement and dynamic air- removal cycles if they are done in the same sterilizer
B. Loads containing implantable devices should be monitored with a BI and Type 5 chemical indicator, and quarantined until the results of the BI results are available
C. If a sterilizer runs cycles at multiple diff erent exposure times, then a BI should be tested using the longer cycle time
D. None of the above
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