EMS Freeze Substitution Kit SAFETY REMINDERS


• The equipment should be used in a fume hood in case there is a leak of osmium-acetone during a run. We suggest doing a trial run with acetone only in the cryovials to make sure that they are sealed correctly.


• When sealing cryotubes that contain frozen fixative and sample, use a warm cap so that the O-ring is flexible and gives a good seal.


• Take care when removing the caps after a FS run because there is some pressure built up inside the cryotubes and you can spray osmium/acetone on your hands if you are not careful. Cover the cap with a piece of lab tissue when removing and wear gloves.


Ordering Information EMS 003 Heater Block EMS 002 Ice Chest


EMS 004 Temperature Probe


EMS 111 Platform Shaker


EMS 005 Cryo Tubes


EMS 006 Data Logger


Catalog No. 34500 34502 34503


34504-B 34504-R 34505 34506 34507


Description


EMS Freeze Substitution Kit (available with or without Shaker – please specify) EMS 111 Platform Shaker (please specify 115 or 220 Volt) EMS 002 Ice Chest


EMS 003 Heater Block, 12mm EMS 003 Heater Block, 13mm EMS 004 Temperature Probe EMS 005 Cryo Tubes EMS 006 Data Logger


Acknowledgements: 1.


References:


McDonald, K, Webb, R. (2011) Freeze Substitution in 3 hours or less. J Microscopy 243, 227-233 Dubochet, J. (2007). The physics of rapid cooling and its implications for cryoimmobilization of cells. Meth. Cell Biol. 79, 7-21.


Buser, C., & Walther, P. (2008). Freeze substitution: the addition of water to polar solvents enhances the retention of structure and acts at temperatures around -60˚C. J. Microsc. 230(2), 268-277.


Quantity Kit


each each each each each each each


K.L. McDonald* and R.I. Webb** * Electron Microsocpe Laboratory, University of California , Berkley, CA and ** Centre for Microscopy an Microanalysis, University of Queensland, Queensland, Australia


Electron Microscopy Sciences In PA: (215) 412-8400 • Toll-Free (800) 523-5874 Fax (215) 412-8450 or 8452 • email: sgkcck@aol.com or stacie@ems-secure.com • www.emsdiasum.com


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