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Digital Staining Discussion


The cell is considered to be the smallest and most basic living entity. It is therefore crucial to explore and analyze it in order to understand the basis of life. The ability to see the cell without killing it, in 3D, and to identify its interior parts in color opens new avenues to further explore cells. Eventually, researchers will be able see and measure the impacts of drugs and stimuli on the cell.


Nanolive uses harmless light, which allows the researcher to “see” directly inside living cells in 3D, without any special preparation and without altering the cell integrity. It shows the cell from inside in real time thereby allowing observation of dynamics taking place within it. T us, the internal pressures and external interactions of cells can be studied in a user-friendly way on mobile devices. Researchers also can upload their cell images to a dedicated cloud library, which will help them compare their data with pre-existing acquisitions from around the globe. T e technology behind this method is quite complex. Holography off ers a new means of probing cells in their native environment that is label-free, non-invasive, manipu- lation-free, and interference-free. Rotational scanning allows real 3D reconstruction, noise robustness, and a resolution enhancement. T e balanced combination between the high resolution (200 nm) of the system and the high sensitivity for diff erent refractive indices (increments of 0.001) opens opportunities for seeing living cells with nanometric details.


It allows measurement of cellular processes in situ with real-time kinetics, enabling multi-parameter analysis at single- cell and sub-cellular scale.


Because the device does not require special sample preparation (no chemicals, no staining), and thanks to its straightforward handling, the 3D Cell Explorer gives researchers a head start for many applications: in-vitro fertilization, cell division, cell diff erentiation, bacteria identifi cation, cell death, etc. Perhaps the most exciting application is the potential for monitoring the eff ect of drugs on patient-specifi c cells as an important part of the development of personal medicine.


Conclusion Described here is a new technique for the analysis and


diff erentiation of cell components without the need for conven- tional staining. Changes in refractive index within the cell itself provide the contrast for labeling various organelles with high precision and at a high resolution. T e method can be applied to the 3D examination of living cells under various external stimuli or drugs.


References [1] Cotte et al ., Nature Photonics 7 ( 2013 ) 113 – 17 . [2] D Gabor , Nature 208 ( 5009 ) ( 1965 ) 422 – 23 . [3] Cotte et al ., J Biomed Opt 16 ( 10 ) ( 2011 ) 106007 . [4] AJ Devaney , Opt Lett 6 ( 1981 ) 374 – 76 . [5] Y Sung et al ., Opt Express 17 ( 2009 ) 266 – 77 .


2015 July • www.microscopy-today.com


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