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Cross reactivity can be a problem when working with primary antibodies sourced from animal species that are evolutionary closely related to the source of tissues or cells that antigens are to be detected in.
An example is using a mouse monoclonal on rat tissue. With many monoclonal antibodies of mouse origin and some of rat, and the prevailing animal cells and tissues being used in studies likewise originating from rat or mouse, it often happens that cross reactivity occurs, leading to false positive results.
A second problem that derives from cross-reactivity occurs in double labeling, when using primary antibodies from closely related animal sources. With this in mind, Aurion has developed two series of new conjugates:
Goat-anti-Mouse IgG (H+L)xRat (cross adsorbed against Rat immunoglobulins) Particle Size
EM-grade Ultra Small EM-grade 10nm
Cat # 25900 25902
Size(ml) Cat # Size(ml) 1.5 2.5
Goat-anti-Rat IgG (H+L)xMouse (cross adsorbed against Mouse immunoglobulins) Particle Size
Secondary antibodies have been thoroughly purified using solid phase technique. These purified antibodies allow detection of antigens with mouse monoclonal antibodies in rat tissue or rat monoclonal antibodies in mouse tissue, without causing interfering binding to either endogenous mouse or rat immunoglobulins
The antibodies can be used for double labeling as well when both mouse and rat primary antibodies are used with other than mouse or rat tissue.
Cross adsorption against closely related species may result in epitope recognition with reduced binding force and lower labeling density.
EMS and Aurion would like to inform you that the strongest labeling intensity is usually achieved with the least refined antibodies. Users are advised to take this into account when choosing a product from the Aurion range of anti-mouse or anti-rat conjugates. In case of doubt, our technical staff will gladly assist you in making the best choice.