HotStart-IT.
®
Less Heat. No DNA Damage.
We couldn’t be more specific.
USB’s novel hot start PCR method requires no extensive heat denaturation step. The result is no damage
to precious samples with increased specificity and yield.
HotStart-IT
®
Taq DNA Polymerase Benefits
• Based on primer sequestration–a novel method High Sensitivity
developed by USB scientists • Detects <10 target copies
• Unique protein binds primers to prevent mispriming
• Linear dynamic range of 7–8 orders of magnitude, R
2
≥ 0.95
• Primers are released during heat denaturation
Ease of Use
• Multiple instrument compatibility for real-time PCR
Increased specificity of HotStart-ITIncreased specificity of HotStart-IT
TM®
Real-time PCR from 10
8
copies down to a single copy target using HotStart-IT
®
Taq DNA Polymerase
Taq DNA Polymerase
1 ng DNA
without with
HotStart-IT HotStart-IT
M– –++
10
8
10
6
10
4
10
2
1
numb, 306 bp
e.com/elab
primer-dimers
Results clearl
Results
y demonstra
clearly
te a shift from predominantl
demonstrate a shift
y
primer-dimers to the specific target when HotStart-IT
from predominantly primer-dimers to
®
the specific target when HotStart-IT
TM

.scientistliv
is included in the reactions.
is included in the reactions.
www

at
Now in Europe
✔
or
USB Europe GmbH: Call +49(0)76 33 - 933 40 0 or visit www.usbweb.de
43
Hauptstrasse 1 79219 Staufen, Germany
cle
USBcustomerserveurope@affymetrix.com USBtechsupporteurope@affymetrix.com
Cir
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