14 CEll CulTurE
Three-dimensional cell culture
matrices offer superior results
Every day scientists
are one step closer to
discovering safer and B
reakthroughs in research, especially Many cell-based assays used in important
in regenerative medicine, which research and screening laboratories today are
is promising cures for numerous not representative of complex in vivo cellular
devastating diseases and injuries, are behaviours simply because they are not being
more effective drugs, and bringing excitement and pressure to the scientific performed in in vivo like environments.
to replacing damaged or
community. The potential for benefits in drug Culturing cells in two-dimensional
diseased human organs
discovery and tissue regeneration is unlimited. environments does not represent cell morphology,
and tissues. Simon
The question in most cases is not whether growth rates, contact geometries, transport
Nikolay reports.
the promise will be fulfilled, but rather when, properties, and numerous other cellular functions
and who will make these discoveries a reality to that are characteristic of the behavior of the cells
Chaque jour, les
patients. in living tissues.
scientifiques font un pas
This growing pressure is demanding rapid HydroMatrix Peptide Hydrogel offered by
vers la découverte de and reliable results from the researchers who are Sigma-Aldrich is a synthetic peptide nanofibre
médicaments plus sûrs
admitting that cell culture techniques are lagging scaffold, which self-assembles into a highly cross-
et plus efficaces et vers
significantly behind the fast developments and linked three-dimensional Hydrogel for use in three-
le remplacement des
breakthroughs of other areas of research. dimensional cell cultures.
organes et tissus humains
endommagés ou malades.
Now, the time has come for cell-based What makes HydroMatrix Peptide Hydrogel
Compte-rendu de Simon
systems to progress and make significant superior to other three-dimensional systems
Nikolay.
contributions simply by switching from offered is the fact that it is a fully synthetic
two-dimensional to three-dimensional (3D) material with no possible source of adventitious
environments in vitro, in which cells are grown agents due to animal-derived matter, allowing
Wissenschaftler kommen and studied. researchers to worry about more important things.
der Entdeckung sichererer
Growing cells on flat
und effektiverer
surfaces seems artificial and
Arzneimittel und dem
unnatural since it does not
Ersatz beschädigter oder
erkrankter menschlicher
bear a resemblance to in vivo
Organe und Gewebe jeden
architectures where cells
Tag einen Schritt näher.
flourish at their best.
Ein Bericht von Simon
Three-dimensional
Nikolay. cell culture scaffolds are a
better representation of the
natural environment that is
experienced by the cells in
Fig.1. Rat neural stem cells (NSC’s) cultured on three surfaces.
the living organism. Reflecting
NSC’s grew poorly on tissue culture plastic (A) and slightly better
natural conditions allows for
in poly-L-lysine/laminin-coated plates (B). NSC’s demonstrated
intercellular interactions with
excellent growth on HydroMatrix peptide hydrogel 05 per cent
more realistic biochemical and
(w/v) (C).
physiological responses.
In a three-dimensional environment, cells Common challenges among researchers doing
behave and respond more like they would in cell culture in three-dimension is that different
vivo to internal and external stimuli, such as cell types have different microenvironments,
changes in temperature, pH, nutrients absorption, which means they require different culture
transport, and differentiation. conditions, growth factors, media, and certainly
The power of regenerative medicine provides different matrices.
the means to understanding numerous intriguing Also, different assays, such as expansion
cellular progressions such as development, aging, or differentiation, require different cell culture
and tissue rejuvenation. conditions.
The only way to understand these fascinating Most three-dimensional systems offered have
processes in biology is to study model systems in fact generic or defined environments, which
that truly resemble those in the organism, making do not accommodate most cells. By adjusting
three-dimensional cell culture imperative. the concentration of the HydroMatrix solutions,
www.scientistlive.com
Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44