William Sutherland


plants than animals. Because of this, matK gene information needs to be supplemented by data from another gene. Although studies utilizing trnH-psbA genes that


share similar


characteristics to matK showed promise (when sequencing of matK and trnH-psbA was utilized involving plants of the nutmeg family (Myristicaceae) the effective range for correct identification rose to approximately 95%), a panel of 52 leading barcoding scientists opted on using the ribulose-bisphosphate carboxylase (rbcL) gene (also located in plant chloroplasts) outlined in a 2009 paper published in Proceedings of the National Academy of Sciences as reported by Daniel Cressey, DNA barcodes for plants a step closer (Nature, 27 July 2009), to effectively complete the barcode for the 10% group.


While discovery of the phylogenetic usefulness of the matK gene is relatively novel, studies indicating the phylogenetic usefulness of the rbcL gene date back as far as 1986 when Jane Aldrich, Barry Cherney, Ellis Merlin and Jeff Palmer reported in Nucleic Acids Research that sequencing of rbcL genes showed petunia and tobacco and alfalfa and peas are 97.3% and 94.1% genetically identical when comparing their bps.


Additional studies, to name two, added further evidence of the phylogenetic usefulness of the rbcL gene. One, reported by Mitsuyasu Hasebe, Tomokyuki Omori, Miyuki Nakazawa, Toshio Sano, Masahiro Kato, and Kunio Iwatsuki in rbcL Gene sequences provide evidence for the evolutionary lineages of leptosporangiate ferns (Proceedings of the National Academy of Sciences, June 1994) utilized PCR-amplified rbcL fragments in 58 species of leptosporangiate ferns, which belong to the pteridophyte class (vascular plants that produce spores to reproduce in lieu of flowers and seeds) which has the longest evolutionary history of any vascular land plant (consequently enduring the greatest loss of plylogentically useful data) to capture their evolutionary links. The other, reported by Hiroaki Setoguchi, Takeshi Asakawa Osawa, Jean-Christophe Pintaud, Tanguy Jaffré, and Jean-Marie Veillon in Phylogenetic


95


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