William Sutherland


May 2010)), which is present in the mitochondrial DNA of every multi-cellular organism, scientists are able to readily determine phylogeny (identification) on a molecular level and store it in databases for easy retrieval. Per P.M. Hollingsworth, DNA bar- coding plants in biodiversity hot spots: Progress and outstanding questions (Heredity, 9 April 2008) "DNA bar-coding is now routinely used for organismal identification" in animals and "has contributed to the discovery of new species."


However, per Mark W. Chase, Nicolas Salamin, Mike Wilkinson, James M. Dunwell, Rao Prasad Kesanakurthi, Nadia Haidar, and Vincent Savolainen, Land plants and DNA barcodes: short-term and long-term goals (Philosophical Transactions Of The Royal Society, 2005) this has not been the case with plants until recently since their CO1 gene does not have the ability to serve as a barcode gene and because they "have had the reputation of being problematic for DNA bar-coding" due to "low levels of variability" and lack of variation in "plastid phylogenetic markers." This view prevailed until 2008 when a team led by Dr. Vincent Savolainen of Imperial College London's Department of Life Sciences and The Royal Botanic Gardens, Kew, studied the functionality of the megakaryocyte-associated tyrosine-protein kinase (matK) gene located in the intron of trnK chloroplast genes found in plant leaves. Their research found that the matK gene (which "contained significant species-level genetic variability and divergence, conserved flanking sites for developing PCR (polymerase chain reaction, a process that enables scientists to produce millions of copies of a specific DNA sequence in about two hours while bypassing the need to use bacteria to amplify DNA) primers for wide taxonomic application, [and] a short sequence length... to facilitate... DNA extraction and amplification") as reported by W. John Kress and David L. Erickson, DNA barcodes: Genes, genomics, and bioinformatics (PNAS. Vol. 105, No. 8. 26 February 2008) and in Polymerase Chain Reaction (PCR) (Gene Almanac. Dolan DNA Learning Center and Cold Spring Harbor Laboratory, Inc. 2009) could be used to differentiate between at least 90% of all plants, including


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