MIDSCI
Agarose from MIDSCI - AMRESCO
Electrophoresis


A g a r o s e LeNders

• Superior Background & Clarity
• Convenient & Ready-to-use Agarose Blends with TBE & TAE Already Added
• Just Add Water, Heat & Pour the Gel

Agarose I™/ TBE and Agarose I™/ TAE powder blends have TBE or TAE buffer already mixed in with the
agarose at a 1X concentration. This unique product is not only easy to use, but also provides excellent
clarity and low background for optimal electrophoresis results.

1 2 3
PRODUCT DESCRIPTION BUFFER CODE SIZE SEE PAGE
Agarose Blenders™ 0.8% TBE K669-50G 50 g 1 9
AMRESCO’s PCR DNA Marker™
(code:E854) w a s separated in four l a ne s
Agarose I™/TBE Blend 0.8%

K669-100G

100 g


of a 2.0% gel of (1) AMRESCO Agarose
Agarose Blenders™ 1.0% TBE K675-50G 50 g 1 9 I™ (2) A competitor agarose and (3)
Agarose I™/TBE Blend 1.0%

K675-100G

100 g


AMRESCO’s Agarose I™/TAE Blend and
stained with SYBR
®
Green I.

Agarose Blenders™ 1.5% TBE K677-50G 50 g 1 9

Agarose I™/TBE Blend 1.5%

K677-100G

100 g


Agarose Blenders™ 2.0% TBE K678-50G 50 g 1 9 A


Agarose I™/TBE Blend 2.0%

K678-100G

100 g



Agarose Blenders™ 0.8% TAE K679-50G 50 g 1 9
Agarose I™/TAE Blend 0.8%

K679-100G

100 g




Agarose Blenders™ 1.0% TAE K680-50G 50 g 1 9

Agarose I™/TAE Blend 1.0%

K680-100G

100 g


Agarose Blenders™ 1.5% TAE K681-50G 50 g 1 9

Agarose I™/TAE Blend 1.5%

K681-100G

100 g




Agarose Blenders™ 2.0% TAE K682-50G 50 g 1 9

Agarose I™/TAE Blend 2.0%

K682-100G

100 g








Optimum Buffer



B
• Speed - Run gels in hour


• Performance - Resolve bands in minutes with sharper resolution

• Quality - Can be run at higher voltages without harming the gel



AMRESCO’s Optimum Buffer is a replacement for traditional TBE and TAE electrophoresis buffers. It

allows for higher voltage runs (2 to 3 times greater without the gel melting), faster running times and


a higher resolution band separation. This 10X buffer is ready for use in gel electrophoresis after diluting

it to a working 1X concentration.

Agarose gels may be prepared using 1X buffer. Optimum™ Buffer is also compatible with standard TBE


and TAE gels, both pre-cast (simply take the pre-cast gel, place it into the electrophoresis chamber, pour

100 bp Ladder (Code: K180-250UL) and 1
in Optimum™ Buffer, load and run) and those made by hand. (TBE and TAE gels run in Optimum™
kb Ladder (K181-500UL) were loaded with
Buffer generally produce better results than when used in their own respective buffers.)
EZ-Vision™ DNA Dye (N313) on 16 x 16

cm 1% agarose gels prepared with either

Optimum™ Buffer or 1X TAE buffer. Each
PRODUCT DESCRIPTION CODE SIZE SEE PAGE
ge l w a s run in its respective buffer. The
Optimum™ Buffer, 10X N115-500ML 500 ml 90
ge l pr e pa r ed with Optimum™ Buffer (A)
was run at a v ol tage of 250V whi l e the
gel prepared with 1X TAE (B) was run at
108V. After 40 m i n utes the bands were
visualized by expos u r e to UV l i ght and w e r e
documented us i ng a Syn ge ne G:BOX Che mi
HR imaging system with a short pa ss filter
(380-650 nm).



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