MIDSCI
Agarose from MIDSCI - AMRESCO
Electrophoresis




Agarose 3:1 HRB
1 2 3 4 5 6 7 8

• High Resolution Blend Optimal for Small Nucleic Acid Fragments &
—2 kb
PCR Products
• Easy to Handle Due to High Gel Strength
• Nuclease & Protease-free



1.8% Agarose 3:1 HRB™ gel, prepared
Agarose 3:1 HRB™ (High Resolution Blend), is a unique mixture of agarose formulated to provide
in 1X TBE buffer. Lanes 1 & 2: PCR DNA
high resolution of small nucleic acids and PCR products. Specially designed for analysis of DNA
Marker™ (Code: 854) Lanes 4 & 5: DNA
fragments less than 1,000 bp, Agarose 3:1 HRB™ displays precise banding patterns without
MicroMarker™ (Code E840) Lanes 7 & 8:
smearing or high background fluorescence. The high gel strength allows for easy-to-handle gels
PCR Marker Plus™ (Code J130).
that are optimal for blotting applications.


Optimal Running Conditions
GEL % OPTIMAL SEPARATION RANGE (bp) RECOMMENDED BUFFER
1.0 500 – 2,000 TBE
2.0 250 – 750 TBE

3.0 125 – 500 TBE

4.0 50 – 250 TBE



PRODUCT DESCRIPTION CODE SIZE SEE PAGE

Agarose 3:1 HRB™ E776-25G 25 g 18
E776-100G 100 g
E776-250G 250 g



Agarose SFR


• Super Fine Resolution: Resolve 238 bp & 242 bp Bands

1 2 3 4


• Low Melting Agarose





• Consistent & Reproducible Results




• Nuclease & Protease-free





• Ideal for AFLP's, STR;s, & Tetranucleotide Repeats


Aga

800 bp—
rose SFR is a high resolution sieving agarose with unsurpassed clarity. DNA bands differing

750 bp—
in s

ize by 2% can be resolved in the range of 200 - 1,000 bp. This agarose is suitable for the

700 bp—

analysis of AFLP’s (Amplified Fragment Length Polymorphisms), STR’s (Short Tandem Repeats)


and tetranucleotide repeats. The low melting temperature of Agarose SFR™ makes it an excellent



medium for analytical and preparative electrophoresis.





Optimal Running Conditions


GEL % OPTIMAL SEPARATION RANGE (bp) RECOMMENDED BUFFER

2.0 500 – 2,000 TAE/ TBE


3.0 100 – 1,200 TAE/ TBE


AMRESC

O DNA markers were stained with
4.0 50 – 500 TAE/ TBE

EZ-Vision™ (N313) and were r e so l v e d on a
5.0 20 – 250 TAE/ TBE

2% Agarose SFR™ gel prepared in 1X TAE

buffer. Lanes 1 & 2: 100bp Ladder (K180-

250UL) Lanes 3 & 4: PCR DNA Marker
PRODUCT DESCRIPTION CODE SIZE SEE PAGE
PLUS™ (J130-250UL).
Agarose SFR™ J234-25G 25 g 18
J234-100G 100 g
J234-250G 250 g




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