IBI GEL/PCR DNA FRAGMENT
Efficient Gel Extraction and PCR Clean-up
The Gel/PCR DNA Fragments Extraction Kits are designed to recover or
concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction,
or any other enzymatic reaction. This method uses a chaotropic salt and
guanidine thiocyanate to dissolve the agarose gel and denature the enzymes.
The DNA fragments in the chaotropic salt are bound to the glass-fi ber matrix
of the spin column. After washing off the contaminants, the purifi ed DNA
fragments are eluted by low salt elution buffer or water. Salts, enzymes, and
unincorporated nucleotides can be effectively removed from the reaction
mixture without phenol extraction or alcohol precipitation.
Order Number Description Size Price
IB47010 Gel/PCR Fragment Extraction Sample Kit
4 preps
$7.00
IB47020 Gel/PCR Fragment Extraction Kit
100 preps
$108.00
IB47030 Gel/PCR Fragment Extraction Kit
300 preps
$317.00
IB47040 4 x 96-Well PCR Clean-Up Kit
4 plates
$427.00
IB47050 10 x 96-Well PCR Clean-Up Kit 10 plates $973.00
IBI HIGH-SPEED
PLASMID MINI KITS
Fast and Economical Plasmid Minipreps
The IBI High-Speed Plasmid Mini Kits are designed for the rapid isolation of plasmid or cosmid DNA from 1-4ml of
bacterial cultures. The modifi ed alkaline lysis method and RNAse treatment are used to create cleared cell lysate with
minimal genomic DNA and RNA contaminants. In the presence of chaotropic salt, the plasmid DNA in the lysate binds
to the glass fi ber matrix in the spin column. The purifi ed plasmid DNA is eluted by a low salt elution buffer or water.
This procedure does not require DNA phenol-extraction or alcohol precipitation.
Order Number Description Size Price
IB47100 MINI High-Speed Plasmid Sample Kit
4 preps
$7.00
IB47101 MINI High-Speed Plasmid Kit
100 preps
$97.00
IB47102 MINI High-Speed Plasmid Kit
300 preps
$258.00
IB47150 2 x 96-Well Plasmid Kit
2 plates
$252.00
IB47151 4 x 96-well Plasmid Kit
4 plates
$469.00
IB47152 10 x 96-Well Plasmid Kit 10 plates $927.50
IBI MIDI/MAXI PLASMID KITS
Ultra-Pure and Large Volume Plasmid Preparation
IBI’s Midi/Maxi Plasmid Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial
cultures. In this process, the modifi ed alkaline lysis method and RNAse treatment are used for creating cleared cell
lysate with minimal genomic DNA and RNA contaminants. Plasmid DNA is bound to the column, washed, and eluted
with a high salt buffer. A fi nal isopropanol precipitation results in ultra-pure plasmid DNA suitable for numerous
downstream applications.
Order Number Description Size Price
IB47110 MIDI Plasmid Sample Kit
2 preps
$24.50
IB47111 MIDI Plasmid Kit
25 preps
$234.00
IB47120 MAXI Plasmid Sample Kit
2 preps
$49.00
IB47121 MAXI Plasmid Kit
10 preps
$227.00
IB47122 MAXI Plasmid Kit 25 preps $433.00
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MMidsci_QckShpGuide_09_Pg21-30.in24 24idsci_QckShpGuide_09_Pg21-30.in24 24 112/23/08 3:09:59 PM2/23/08 3:09:59 PM
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