| research report | ARTICLE ■ First prp treatment.
Visit 1 (V1) between Day 21 (D21) and Day 28 (D28) ■ subjective skin evaluation ■ Biometric measurements ■ Discussion of possible side-effects ■ second prp treatment.
Visit 2 (V2) between Day 90 (D90) and day 120 (D120) ■ subjective skin evaluation ■ Biometric measurements ■ report of side-effects ■ end of the study.
Treatment the treatment protocol consisted of injecting 4 cc of prp. the injections were made to the lower part of the face (Figure 5), and to designated intra-dermal points (black crosses in Figure 5). the injections were intended to cover the measurement zones. the preparation of the prp was made in accordance
with the operating instructions of the kit manufacturer. the blood was collected and spun in a centrifuge at 1200 g (4000 rpM) for 7 minutes. after discarding the platelet poor plasma phase, the buffy layer was resuspended in approximately 4 mL of plasma fluid. Following resuspension, the filter sleeve was used to assist in the transfer into of the prp into a syringe fitted with a with 30 G needle. No calcium chloride was added to the prp prior to reinjection. all measurements were made with the following
conditions: ■ No make-up in the measurement area ■ any make-up removal was performed at least 1 hour prior to measurement
PRP
Surface Length R G B
Luminance a b
Anistropy Ra Rv Rp Rt
TEWL
Temperature Hydration
V0 39.21 6.51
175.16 147.30 123.22 62.94 6.83
16.66 28.96 15.28 40.15 79.18 119.27 14.09 28.03 57.3
The
treatment protocol
consisted of
injecting 4 cc of PRP. The
injections were made to the
lower part of the face and to designated
intra-dermal points.
Figure 5 Zones of injection and measurement
Figure 6 4-week results. R, G, B and Luminance, a, b are calorimetric parameters; Rt, Rp, Rv, Ra are micro-relief parameters
V1–V0 Var 1 -1.67 -0.21 5.92 8.24 8.84 2.99
-4.26 -3.15 3.38 5.6 7.17
4.76
-0.99 -0.84 -6.98 -0.72 -0.88 -3.82 -4.17 -1.16 -0.14 -3.48
-14.55 -5.02 -24.1 -4.71 -2.18 -4.82 -3.49 -8.23 -0.51 -6.07
-14,55 -5,02 -24,10 -4,71 -2,18
-4,82 -3,49
-8,23 -0,51 -6,07
■ No cosmetic or beauty products should be applied within the 2 hours prior to measurement ■ the zone of the measurement was defined by the intersection of a line from the ear to the labial fold and another line from the bottom of the ear to the nostril (green circle in Figure 5).
No modifications to normal beauty care routines were allowed between visits. patients were not permitted to
Variation on Day 30
-4,26 -3,15
3,38
5,60 7,17
4,76
Surface Length R G B
Luminance a b
Anisotropy Ra Rv Rp Rt
TEWL Temperature Hydration
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