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a standard index. the results are expressed as a percentage and are categorised as: ■ over 50% is considered normal for the skin ■ From 30–50% the skin is classified as tending towards dry or ‘medium’ ■ Under 30% the skin is considered dry. Following marked swelling of the skin, a number of


microlines on the surface of the skin can be observed. the projection and distribution of these microlines two-dimensionally in all directions (360°) is indicative of young skin. a child will have lines oriented homogeneously in all directions, which is reflective of good skin elasticity. the older the skin, the more these lines will change orientation and become parallel. anisotropy measures the distribution of microlines in 360°, and is expressed as a percentage. If the index reading is low, the microlines are at a 360° orientation (young skin), but if high, the microlines tend to be parallel (elderly skin). a decrease in this index is indicative of the degree to which a given treatment has had an anti-ageing effect. It is undoubtedly the most significant parameter when evaluating the anti-ageing effect of a beauty product or a morpho-aesthetic therapy. teWL is a non-invasive measurement of the amount of


Figure 1 MyCells tubes


Materials and methods MyCells Kits Blood withdrawal, preparation of platelet concentrate and its subsequent injection were performed using the Mycells autologous platelet preparation Kit. the proprietary tubes, ppt-I and ppt-II (Figure 1), as well the necessary syringes and needles, are also included in the kit. the Mycells Kits were supplied by Kaylight corporation, who designed them specifically to create a high yield and viable prp.


IntuiSkin Professional WorkStation the measurement of skin parameters was made using an integrated work station supplied by Intuiskin (Figure 2). the validity of this equipment for the evaluation of biometric measurements has already been established13, 14


.


the Workstation includes a video camera with 50x lens, UsB sensors that are fully temperature and humidity compensated, and a Windows Xp pro-based portable computer. the system is capable of making simultaneous measurements of hydration, transepidermal water loss (teWL), and surface temperature.


Objective measurements the temperature of the stratum corneum is recorded along with a measurement of the ambient temperature. Both are expressed in degrees celsius. skin hydration was made after compensating for the ambient humidity level. the hydration was expressed by


32 ❚ November/December 2011 | prime-journal.com


Figure 2 The IntuiSkin WorkStation


epidermal water either lost or evaporated. It is expressed in grams per hour per square metre of cutaneous surface. the range of the measurement is 0–100 gram/m²/hr. teWL reflects the integrity of the stratum corneum, which is the barrier the body has in preventing water loss. the measurement method uses two solid-state microsensors placed at two different positions. the difference between the vapour concentrations at these two points allows a ‘gradient’ to be calculated. Values generally range from 10 to 20 and are considered most accurate when the indicator is below 10. higher readings may indicate that the ability of the skin to provide a barrier to water loss has been compromised. pigmentation is measured using the probe and expressed in levels of red, green and blue (rGB)


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